Depth Filtration time :marseyscientist: :marseyvirus:

https://i.rdrama.net/images/17084780220137956.webp

My last filter sucked butt so I'm happy this one is functioning well.

23
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are you making moonshine?

:#marseymoonshine:

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No, viruses (AAV) :marseycovidscare:

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Covid-24 here we goooo!

:#marseyletsgo:

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>tween 20

Calling it that always made me uncomfortable. I prefer polysorbate 20.

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The bottle says Tween 20 :marseyretardchad:

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If you are using tween before 26 years old, you're a p-do

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:marseyceiling: are yall making super anthrax in there

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That's not a virus :marseymad:

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>Tween 20

isnt that what muslims get when they blow up?

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T W E E N

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You doing process development? I imagine IPS = in process sample or something.

I have some colleagues struggling with depth filtration and tfdf right now.

What are you making? Antibodies, virus, vesicles, etc?

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Purifying AAV2; my thesis work pertains to AEX so I have to develop every unit op beforehand. Annoying af since my affinity columns are acting extremely weird and my running theory is that something is occurring upstream that's inhibiting binding

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Based fellow chromatographer :gigachad3:

Is the AEX work related to separation based on empty/full capsid?

I haven't ran affinity since I used to work on Protein A columns. Are these like the POROS AAVX ones that thermo sells? What kind of weirdness?

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:#marseygrizz2:

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>Tween 20

Twink teen?

Teen twink?

20 of them??

:marseyhmmm:

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OP just wants some spare parts, is that really too much to ask?

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